Table 1.

Description of the Acute Immunogenicity Studies

immunogenicity study	administered particle	particle relative dose (relative number concentration)	euthanasia time point after particle injection	statistics
effect of particle size and dose	μNETs, nNETs	1×, 0.5×, 0.25×	2 h	•Brown–Forsythe one-way ANOVA, and Welch one-way ANOVA followed by unpaired Welch’s t-tests (two-tailed unless otherwise noted) •one-tailed unpaired Welch’s t-test for comparisons to LPS, and comparisons between doses of the same construct
time-dependent cytokine response	μNETs, nNETs	1×	2, 4, 6 h	•Brown–Forsythe one-way ANOVA, and Welch one-way ANOVA followed by unpaired Welch’s t-tests (two-tailed unless otherwise noted) •one-tailed unpaired Welch’s t-test for comparisons to LPS (except comparisons between LPS at different time points)
effects of nNET functionalization with folate or Herceptin	FF-nNETs, HF-nNETs	1×	4 h	•Brown–Forsythe one-way ANOVA and Welch one-way ANOVA followed by unpaired two-tailed Welch’s t-tests
effects of dual injections of NETs one week apart	μNETs, nNETs	0.5×	2 h after second injection	•unpaired two-tailed Welch’s t-tests