Skip to main content
. 2023 Jan 17;33(3):201–214. doi: 10.1038/s41422-022-00775-y

Fig. 3. ZBP1 promotes SARS-CoV-2-induced inflammatory signaling and lung damage.

Fig. 3

a–j Zbp1−/− and wild-type mice were divided into three groups: Ad5-hACE2 non-transduced and non-infected (n = 3), transduced but non-infected (n = 3), transduced and infected (n = 6). Mice were intranasally transduced with Ad5-hACE2 (2.5 × 108 PFU) and infected with SARS-CoV-2 (1 × 105 TCID50) at 5 days post transduction. Lung samples were harvested at 2 dpi. a Relative level of indicated genes was analyzed with qRT-PCR and normalized to non-transduced and uninfected wild-type controls. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t-test. **P < 0.01; ns, no significance. b The histopathological changes in lung were evaluated by H&E staining. Scale bars, 1000 μm and 50 μm in enlarged image. Inflammatory cell infiltration (green arrow) and alveolar septa expansion (red arrow) were observed in the lung sections. Immunostaining of CD45 (c), CD68 (e), MPO (g) and CD3 (i) representing the infiltration of leukocytes, macrophages, neutrophils and T cells, respectively. c, i Scale bars, 50 μm and 10 μm in the enlarged image. e, g Scale bars, 50 μm. d, f, h, j Quantitative analysis of positive cells was performed with ImageJ for c, e, g, i, respectively. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t-test. **P < 0.01; ****P < 0.0001.