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. 2023 Jan 17;33(3):201–214. doi: 10.1038/s41422-022-00775-y

Fig. 5. The scaffolding function of RIPK3 is critical for SARS-CoV-2-triggered inflammatory chemokine production in the lung.

Fig. 5

a Ripk3−/−, Mlkl−/− or wild-type C57BL/6 mice (n = 4) were intranasally transduced with Ad5-hACE2 (2.5 × 108 PFU) and infected with SARS-CoV-2 (1 × 105 TCID50) at 5 days post transduction. Ad5-hACE2 transduced but uninfected wild-type C57BL/6 mice (n = 3) were used as the control. Lung samples were harvested at 2 dpi. Relative mRNA level of indicated genes was analyzed with qRT-PCR and normalized to samples from transduced but uninfected wild-type mice. b The effect of GSK872 on SARS-CoV-2-induced upregulation of cytokines and chemokines in the lung. BALB/c mice were intranasally infected with the mouse-adapted virus (WBP-1, 1 × 104 TCID50) and treated with vehicle (n = 6) or GSK872 (10 mg/kg/d) (n = 6) by intraperitoneal injection. Mice were euthanized at 5 dpi and lung samples were harvested for qRT-PCR determination. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t-test. *P < 0.05; ***P < 0.001; ns, no significance.