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. 2023 Jan 12;16(5):dmm049742. doi: 10.1242/dmm.049742

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 7. — Mediation analysis of the microbiome on changes to EBD, cytokines, SCFAs and eicosanoids. (A) The three outcomes, PGD2, PGE2 and valeric acid, to be mediated by changes in the microbiome. Exposure is CPB, the mediator is the microbiome, and the outcomes are listed above. (B) The three markers of EBD, FABP2, claudin-2 and claudin-3, depicting no statistically significant mediation effect of the microbiome on the changes in EBD. CNT, control; FABP2, fatty acid-binding protein 2. Blue lines indicate the association between the microbiome and individual biomarkers using principal component axis 1, and shaded gray areas represent the 95% confidence intervals.