Fig. 8.

Traumatic noise exposure increases immunolabeling for CaMKKβ in OHCs of CBA/J mice. A Immunolabeling for CaMKKβ (red) in OHCs of the basal turn appears stronger when processed 24 h after completion of the PTSN exposure (PTSN 24 h post) compared with unexposed controls. The enlarged OHCs allow for better visualization of punctate labeling for CaMKKβ; green: phalloidin-stained OHCs. Scale bar = 10 µm. B Semi-quantitative analysis of relative CaMKKβ labeling (grayscale) in OHCs confirms a significant increase when examined at 24 h, but not at 1–3 h after PTSN. Data are presented as means + SD; the number of animals in each group is indicated in the bar graph, *p < 0.05. C Immunoblots using whole cochlear tissue homogenates reveal specificity for CaMKKβ but show no change in band densities for CaMKKβ examined 1–3 h after the noise exposure compared with unexposed controls. GAPDH serves as the loading control. The molecular weights are indicated to the right of the bands. D Quantification of the band densities confirmed no difference between the two groups. The number of animals in each group is indicated in the bar graph, ns not significant