Figure 5. Transcriptomic diversity of human hippocampal microglia across the postnatal lifespan.

(A and B) UMAP of integrated data highlighting microglia (A), which were sub-clustered and visualized in UMAP colored by subpopulation and generic marker expression (B).

(C-E) Characteristics of microglia subpopulations and their abundance across ages. Heatmap (C) and dot plots (D and E) similar as in Figures 2C-E.

(F and G) Sample confocal images (F) and quantification (G) of CD83⁺ cells among all IBA1⁺ microglia in the human hippocampus across ages. Asterisks and arrowheads indicate CD83⁺ and CD83^- cells among IBA1⁺ cells, respectively. Insets boxed in orange and cyan colors show enlarged view of representative IBA1⁺ cells that were CD83⁺ and CD83^-, respectively. Scale bars, 10 μm (F). Box plot similar as in Figure 2G (n = 4 subjects per stage) (G).

(H and I) Assessing hPSC-derived microglia in three query datasets (Popova et al., 2021; Svoboda et al., 2019) with our in vivo reference map (H). UMAPs (H) and bar plots (I) similar as in Figures 2H and 2I.

See also Figure S4, Tables S2 and S3.