FIG. 2.

Antigenicity of the crude extracts of M. avium 2447 proteins. Single-cell suspensions were prepared from spleens of mice infected for 15 (T15), 30 (T30), 60 (T60), or 90 (T90) days with M. avium 2447 or noninfected (naive) animals and stimulated in vitro with 4 μg of cytosol, envelope, or culture filtrate proteins per ml. IFN-γ release was quantified by ELISA in the 72-h culture supernatants. Results are expressed as means of values of triplicate samples ±1 standard deviation performed on cells pooled from four mice. Statistically significant differences compared to values for nonstimulated cells are labeled: ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P = 0.00.