Fig. 1. Overview. A new unified protocol and a previously established automated decellularization system were used to derive ECM-based biomaterials from 8 different tissue sources (vocal fold lamina propria (VFLP), supraglottic (SG), lung, heart, skin, muscle, tendon, and meniscus). The resulting ECM scaffolds were further processed into hydrogels. The feasibility of this method was assessed via double stranded DNA (dsDNA) quantification, discovery proteomics, gelation kinetics, rheology, and cytocompatibility assays.