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. 2023 Jan 17;18(1):e0279961. doi: 10.1371/journal.pone.0279961

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© 2023 Agostini et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Different transfection experiments were performed independently with miRNA mimic, or miRNA inhibitor or scramble control. Each experiment was repeated at least 3 times and conducted in triplicate; the results presented in the figures indicate the mean values obtained. SNAP-25 protein concentration (ng/ml) were measured in MO3.13 cells in different time points: 6, 24, 48 and 72 hours after transfection with (a) miR-23a-3p mimic, or inhibitor or scramble negative control by ELISA (a) and by flow cytometry (b); miR-181a-5p mimic, or inhibitor or scramble negative control by ELISA (c) and by flow cytometry (d). SNAP-25 concentration in cells transfected with miR-23a-3p inhibitor was significantly higher compared to that observed in cells transfected with miR-23a-3p mimic or scramble negative control in each time point; SNAP-25 concentration in cells transfected with miR-181a-5p inhibitor was significantly lower compared to that observed in cells transfected with miR-181a-5p mimic or scramble negative control in each time point.