Skip to main content
. 2022 Dec 8;324(2):F138–F151. doi: 10.1152/ajprenal.00052.2022

Figure 1.

Figure 1.

Effect of 11,12-epoxyeicosatrienoic acid (EET) and 14,15-EET on kidney fibrosis following unilateral ureteral obstruction (UUO). A: male C57BL/6 mice were subjected to either UUO or sham operation and then administered with the combination of 11,12-EET + 14,15-EET (3, 6, 7.5, or 15 µg/kg/day of each, using an osmotic pump) for 7 days. Kidneys were subjected to Western blot analysis using anti-α-smooth muscle actin (α-SMA) antibody. B: mice were subjected to either UUO or sham operation and then administered with a single treatment of either 11,12-EET or 14,15-EET (15 µg/kg/day). The kidneys were subjected to Western blot analysis using anti-α-SMA antibody. GAPDH was used as a loading control. Band density was measured using ImageJ software. Data are presented as means ± SD; n = 4. ***P < 0.001; ****P < 0.0001. One-way or two-way ANOVA followed by a Tukey’s post hoc multiple comparison test was used to determine significance. NS, not significant; V, vehicle.