Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| genetic reagent (Drosophila melanogaster) | ppk-GAL4 UAS-mCD8:GFP | Grueber et al., 2007 (https://doi.org/10.1242/dev.02666) | N/A | |
| genetic reagent (D. melanogaster) | Gr28b.c-GAL4 UAS-mCD8:GFP | Xiang et al., 2010 (https://doi.org/10.1038/nature09576) | N/A | |
| genetic reagent (D. melanogaster) | ppk-CD4-tdGFP1b | Han et al., 2011 (https://doi.org/10.1073/pnas.1106386108) | N/A | |
| genetic reagent (D. melanogaster) | ppk-CD4-tdGFP8 | Han et al., 2011 (https://doi.org/10.1073/pnas.1106386108) | N/A | |
| genetic reagent (D. melanogaster) | ppk-CD4-tdTom4a | Han et al., 2011 (https://doi.org/10.1073/pnas.1106386108) | N/A | |
| genetic reagent (D. melanogaster) | Ror4 | Ripp et al., 2018 (https://doi.org/10.1242/bio.033001) | N/A | |
| genetic reagent (D. melanogaster) | ppk-GAL4 | Bloomington Drosophila Stock Center | Stock #: 32079 | |
| genetic reagent (D. melanogaster) | Mhc-GAL4 | Schuster and Davis, 1996 (https://doi.org/10.1016/s0896-6273(00)80,197x) | N/A | |
| genetic reagent (D. melanogaster) | Cg-GAL4 | Bloomington Drosophila Stock Center | Stock #: 7011 | |
| genetic reagent (D. melanogaster) | apts78j wg1 | KYOTO Stock Center | Stock #: 107069 | |
| genetic reagent (D. melanogaster) | wgl-8 cn1 bw1 speck1 | KYOTO Stock Center | Stock #: 107019 | |
| genetic reagent (D. melanogaster) | wg-GAL4 | Bloomington Drosophila Stock Center | Stock #: 83627 | |
| genetic reagent (D. melanogaster) | UAS-RedStinger | Bloomington Drosophila Stock Center | Stock #: 8547 | |
| genetic reagent (D. melanogaster) | UAS-Akt RNAi (BL33615) | Bloomington Drosophila Stock Center | Stock #: 33615 | |
| genetic reagent (D. melanogaster) | UAS-Akt RNAi (v2902) | Vienna Drosophila Resource Center | Stock #: 2902 | |
| genetic reagent (D. melanogaster) | UAS-Ror RNAi | National Institute of Genetics | Stock #: 4926 R-1 | |
| genetic reagent (D. melanogaster) | UAS-wg RNAi | Vienna Drosophila Resource Center | Stock #: 6692 | |
| genetic reagent (D. melanogaster) | UAS-Stat92E RNAi (BL33637) | Bloomington Drosophila Stock Center | Stock #: 33637 | |
| genetic reagent (D. melanogaster) | UAS-hop RNAi | Bloomington Drosophila Stock Center | Stock #: 32966 | |
| genetic reagent (D. melanogaster) | UAS-upd2 RNAi (5988 R-1) | National Institute of Genetics | Stock #: 5988 R-1 | |
| genetic reagent (D. melanogaster) | UAS-hop | Bloomington Drosophila Stock Center | Stock #: 79033 | |
| genetic reagent (D. melanogaster) | UAS-myrAkt | Bloomington Drosophila Stock Center | Stock #: 50758 | |
| genetic reagent (D. melanogaster) | UAS-wg.H.T:HA1 | KYOTO Stock Center | Stock #: 108488 | |
| genetic reagent (D. melanogaster) | 10XSTAT92E-GFP | Bloomington Drosophila Stock Center | Stock #: 26197 | |
| genetic reagent (D. melanogaster) | UAS-InR RNAi (BL31594) | Bloomington Drosophila Stock Center | Stock #: 31594 | |
| genetic reagent (D. melanogaster) | UAS-InR RNAi (BL51518) | Bloomington Drosophila Stock Center | Stock #: 51518 | |
| genetic reagent (D. melanogaster) | UAS-grnd RNAi | Vienna Drosophila Resource Center | Stock #: 43454 | |
| genetic reagent (D. melanogaster) | UAS-wgn RNAi | Vienna Drosophila Resource Center | Stock #: 9152 | |
| genetic reagent (D. melanogaster) | UAS-mth RNAi | Vienna Drosophila Resource Center | Stock #: 102303 | |
| genetic reagent (D. melanogaster) | UAS-babo RNAi | Bloomington Drosophila Stock Center | Stock #: 25933 | |
| genetic reagent (D. melanogaster) | UAS-Tor.TED | Bloomington Drosophila Stock Center | Stock #: 7013 | |
| genetic reagent (D. melanogaster) | UAS-S6k.KQ | Bloomington Drosophila Stock Center | Stock #: 6911 | |
| genetic reagent (D. melanogaster) | UAS-Tif-IA RNAi | Vienna Drosophila Resource Center | Stock #: 20336 | |
| genetic reagent (D. melanogaster) | UAS-Thor RNAi | Vienna Drosophila Resource Center | Stock #: 35439 | |
| genetic reagent (D. melanogaster) | UAS-foxo RNAi | Bloomington Drosophila Stock Center | Stock #: 32427 | |
| genetic reagent (D. melanogaster) | UAS-Alk RNAi (v11446) | Vienna Drosophila Resource Center | Stock #: 11446 | |
| genetic reagent (D. melanogaster) | UAS-Alk RNAi (BL107083) | Vienna Drosophila Resource Center | Stock #: 107083 | |
| genetic reagent (D. melanogaster) | mef2-GAL4 | Bloomington Drosophila Stock Center | Stock #: 27390 | |
| genetic reagent (D. melanogaster) | R38F11-GAL4 | Bloomington Drosophila Stock Center | Stock #: 50014 | |
| genetic reagent (D. melanogaster) | UAS-fz RNAi | Vienna Drosophila Resource Center | Stock #: 43075 | |
| genetic reagent (D. melanogaster) | UAS-fz2 RNAi | National Institute of Genetics | Stock #: 9739 R-1 | |
| genetic reagent (D. melanogaster) | “GAL45-40 UAS-Venus:pm SOP-FLP#42; tubPGal80 FRT2A” | KYOTO Stock Center | Stock #: 109950 | |
| genetic reagent (D. melanogaster) | “w*; FRT2A” | KYOTO Stock Center | Stock #: 106623 | |
| genetic reagent (D. melanogaster) | “y w hs-flp; fz2C2 FRT2A” | Chen and Struhl, 1999 (https://doi.org/10.1242/dev.126.23.5441) | N/A | |
| genetic reagent (D. melanogaster) | UAS-dsh RNAi | Vienna Drosophila Resource Center | Stock #: 101525 | |
| genetic reagent (D. melanogaster) | UAS-bsk.DN | Bloomington Drosophila Stock Center | Stock #: 6409 | |
| genetic reagent (D. melanogaster) | UAS-DAAM RNAi | Vienna Drosophila Resource Center | Stock #: 24885 | |
| genetic reagent (D. melanogaster) | UAS-arm RNAi | Vienna Drosophila Resource Center | Stock #: 7767 | |
| genetic reagent (D. melanogaster) | UAS-norpA RNAi | Bloomington Drosophila Stock Center | Stock #: 31113 | |
| genetic reagent (D. melanogaster) | UAS-Axn RNAi | Bloomington Drosophila Stock Center | Stock #: 31703 | |
| genetic reagent (D. melanogaster) | UAS-Stat92E RNAi (BL31318) | Bloomington Drosophila Stock Center | Stock #: 31318 | |
| genetic reagent (D. melanogaster) | UAS-dome RNAi (v106071) | Vienna Drosophila Resource Center | Stock #: 106071 | |
| genetic reagent (D. melanogaster) | UAS-dome RNAi (BL32860) | Bloomington Drosophila Stock Center | Stock #: 32860 | |
| genetic reagent (D. melanogaster) | UAS-dome RNAi (BL34618) | Bloomington Drosophila Stock Center | Stock #: 34618 | |
| genetic reagent (D. melanogaster) | UAS-upd RNAi (BL33680) | Bloomington Drosophila Stock Center | Stock #: 33680 | |
| genetic reagent (D. melanogaster) | UAS-upd RNAi (v3282) | Vienna Drosophila Resource Center | Stock #: 3282 | |
| genetic reagent (D. melanogaster) | UAS-upd2 RNAi (BL33949) | Bloomington Drosophila Stock Center | Stock #: 33949 | |
| genetic reagent (D. melanogaster) | UAS-upd3 RNAi (BL32859) | Bloomington Drosophila Stock Center | Stock #: 32859 | |
| genetic reagent (D. melanogaster) | UAS-upd3 RNAi (BL28575) | Bloomington Drosophila Stock Center | Stock #: 28575 | |
| genetic reagent (D. melanogaster) | rn-GAL4 | St Pierre et al., 2002 (https://doi.org/10.1242/dev.129.5.1273) | N/A | |
| genetic reagent (D. melanogaster) | UAS-dilp8 | Colombani et al., 2012 (https://doi.org/10.1126/science.1216689) | N/A | |
| genetic reagent (D. melanogaster) | UAS-htl RNAi (v6692) | Vienna Drosophila Resource Center | Stock #: 6692 | |
| genetic reagent (D. melanogaster) | UAS-htl RNAi (BL35024) | Bloomington Drosophila Stock Center | Stock #: 35024 | |
| genetic reagent (D. melanogaster) | UAS-Egfr RNAi | Vienna Drosophila Resource Center | Stock #: 43267 | |
| genetic reagent (D. melanogaster) | UAS-Pvr RNAi | Vienna Drosophila Resource Center | Stock #: 13502 | |
| genetic reagent (D. melanogaster) | UAS-Ddr RNAi | Vienna Drosophila Resource Center | Stock #: 29720 | |
| genetic reagent (D. melanogaster) | UAS-dnt RNAi | National Institute of Genetics | Stock #: 17,559 R-3 | |
| genetic reagent (D. melanogaster) | UAS-drl RNAi | Bloomington Drosophila Stock Center | Stock #: 29602 | |
| genetic reagent (D. melanogaster) | UAS-Eph RNAi | Bloomington Drosophila Stock Center | Stock #: 28511 | |
| genetic reagent (D. melanogaster) | UAS-otk RNAi | Bloomington Drosophila Stock Center | Stock #: 25790 | |
| genetic reagent (D. melanogaster) | UAS-sev RNAi | Bloomington Drosophila Stock Center | Stock #: 31274 | |
| genetic reagent (D. melanogaster) | UAS-btl RNAi | Vienna Drosophila Resource Center | Stock #: 110277 | |
| genetic reagent (D. melanogaster) | UAS-Cad96Ca RNAi | Vienna Drosophila Resource Center | Stock #: 1089 | |
| genetic reagent (D. melanogaster) | UAS-CG10702 RNAi | Vienna Drosophila Resource Center | Stock #: 27052 | |
| genetic reagent (D. melanogaster) | UAS-Drl-2 RNAi | Vienna Drosophila Resource Center | Stock #: 40484 | |
| genetic reagent (D. melanogaster) | UAS-Nrk RNAi | Vienna Drosophila Resource Center | Stock #: 9653 | |
| genetic reagent (D. melanogaster) | UAS-Ret RNAi | Vienna Drosophila Resource Center | Stock #: 107648 | |
| genetic reagent (D. melanogaster) | UAS-tor RNAi | Vienna Drosophila Resource Center | Stock #: 36280 | |
| genetic reagent (D. melanogaster) | UAS-Tie RNAi | Vienna Drosophila Resource Center | Stock #: 26879 | |
| genetic reagent (D. melanogaster) | Gal419-12 | Xiang et al., 2010 (https://doi.org/10.1038/nature09576) | N/A | |
| antibody | anti-Wg (Mouse monoclonal) | Developmental Studies Hybridoma Bank | Cat# 4D4, RRID: AB_528512 | IF (1:15) |
| antibody | anti-Futch (Mouse monoclonal) | Developmental Studies Hybridoma Bank | Cat# 22C10, RRID: AB_528403 | IF (1:20) |
| antibody | anti-phospho-Akt (Rabbit polyclonal) | Cell Signaling | Cat# 9271 S | IF (1:100) |
| antibody | anti-DsRed (Rabbit polyclonal) | Clontech | Cat# 632496 | IF (1:250) |
| antibody | anti-Mouse IgG Alexa Fluor 488 (Goat polyclonal) | Invitrogen | Cat# A11029 | IF (1:1000) |
| antibody | anti-Rabbit IgG Alexa Fluor 488 (Goat polyclonal) | Invitrogen | Cat# A11034 | IF (1:1000) |
| antibody | anti-Mouse IgG Alexa Fluor 546 (Goat polyclonal) | Invitrogen | Cat# A11030 | IF (1:1000) |
| chemical compound, drug | Brewer’s Yeast | MPBio | Cat# 2903312 | |
| chemical compound, drug | Yeast extract | Sigma-Aldrich Fluka | Cat# 70161 | |
| chemical compound, drug | Peptone from casein, enzymatic digest | Sigma-Aldrich Fluka | Cat# 82303 | |
| chemical compound, drug | Glucose | Wako | Cat# 049–31165 | |
| chemical compound, drug | Sucrose | Wako | Cat# 196–00015 | |
| chemical compound, drug | MgSO4 | Wako | Cat# 132–00435 | |
| chemical compound, drug | CaCl2 | Wako | Cat# 031–00435 | |
| chemical compound, drug | agar | Matsuki Kanten | N/A | |
| chemical compound, drug | Agar Purified, powder | Nacalai Tesque | Cat# 01162–15 | |
| chemical compound, drug | propionic acid | Nacalai Tesque | Cat# 29018–55 | |
| chemical compound, drug | butyl p-hydroxybenzoate | Nacalai Tesque | Cat# 06327–02 | |
| chemical compound, drug | 50 x MEM Essential Amino Acids Solution | Wako | Cat# 132–15641 | |
| chemical compound, drug | BSA | Nacalai Tesque | Cat# 01863–77 | |
| chemical compound, drug | Alexa Fluor 488 Phalloidin | Invitrogen | Cat# A12379 | |
| chemical compound, drug | FluorSave Reagent | Calbiochem | Cat# 345789 | |
| chemical compound, drug | Formalin | Nacalai Tesque | Cat# 16222–65 | |
| chemical compound, drug | DAPI | Nacalai Tesque | Cat# 19178–91 | |
| software, algorithm | DeTerm |
Kanaoka et al., 2019 ( https://doi.org/10.1111/gtc.12700) |
N/A | |
| software, algorithm | R | R Core Team | RRID:SCR_001905 | |
| software, algorithm | Fiji | NIH | RRID:SCR_002285 | |
| software, algorithm | Photoshop | Adobe | RRID:SCR_014199 | |
| other | LED desk light | OHM | Cat# ODS-LKL6-W | This LED light was used in Light/dark choice assay |