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. 2022 Dec 13;11:e81976. doi: 10.7554/eLife.81976

Figure 12. Regardless of the tracing methods, flexor and extensor related premotor interneurons distributions are always overlapping.

The correlation and absolute value of hierarchical bootstrapped Hedges’ G coefficient matrix across pooled experiments obtained with different injection strategies exhibits high values of correlation and low effect sizes across different techniques and across the two different muscles (A). The median values of the mediolateral position of premotor interneurons in the dorsal ipsilateral quadrant are similar for all conditions (B).

Figure 12—source data 1. Panel B showing the comparison of median of mediolateral coordinates of extensor (LG or GS) and flexor (TA) in the ipsilateral dorsal quadrant for all the viral tracing techniques used in this paper.

Figure 12.

Figure 12—figure supplement 1. Distribution of motor neurons (triangles) and premotor interneurons (dots) of 3 LG (blue) and TA (yellow) double injections (UCL) of rabies virus in ChatCre/+; Rosa26RΦGT mice.

Figure 12—figure supplement 1.

Figure 12—figure supplement 1—source data 1. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 170427n2(UCL).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 1—source data 2. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 170427n3 (UCL).
Figure 12—figure supplement 1—source data 3. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 170503n6 (UCL).
Figure 12—figure supplement 1—source data 4. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 170427n2 (UCL).
Figure 12—figure supplement 1—source data 5. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 170427n3 (UCL).
Figure 12—figure supplement 1—source data 6. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 170503n6 (UCL).
Figure 12—figure supplement 2. Distribution of motor neurons (triangles) and premotor interneurons (dots) of 2 LG (blue) and TA (yellow) double injections (UoG) of rabies virus in ChatCre/+; Rosa26RΦGT mice.

Figure 12—figure supplement 2.

Figure 12—figure supplement 2—source data 1. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 1577 (UoG).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 2—source data 2. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 1578 (UoG).
Figure 12—figure supplement 2—source data 3. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 1577 (UoG).
Figure 12—figure supplement 2—source data 4. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 1578 (UoG).
Figure 12—figure supplement 3. Distribution of motor neurons (triangles) and premotor interneurons (dots) of 2 LG (blue) and MG (dark blue) double injections (UCL) of rabies virus in ChatCre/+; Rosa26RΦGT mice.

Figure 12—figure supplement 3.

Figure 12—figure supplement 3—source data 1. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 170125n3 (UCL).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 3—source data 2. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 170508n7 (UCL).
Figure 12—figure supplement 3—source data 3. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from MG injection 170125n3 (UCL).
Figure 12—figure supplement 3—source data 4. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from MG injection 170508n7 (UCL).
Figure 12—figure supplement 4. Distribution of motor neurons (triangles) and premotor interneurons (dots) of 4 LG (blue) and MG (yellow) double injections (UoG) of rabies virus in ChatCre/+; Rosa26RΦGT mice.

Figure 12—figure supplement 4.

Figure 12—figure supplement 4—source data 1. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 1579 (UoG).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 4—source data 2. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 1580 (UoG).
Figure 12—figure supplement 4—source data 3. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 1701(UoG).
Figure 12—figure supplement 4—source data 4. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 1702 (UoG).
Figure 12—figure supplement 4—source data 5. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from MG injection 1579 (UoG).
Figure 12—figure supplement 4—source data 6. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from MG injection 1580 (UoG).
Figure 12—figure supplement 4—source data 7. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from MG injection 1701 (UoG).
Figure 12—figure supplement 4—source data 8. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from MG injection 1702 (UoG).
Figure 12—figure supplement 5. Distribution of motor neurons (triangles) and premotor interneurons (dots) of 2 TA (yellow) and PL (dark orange) double injections (UCL) of rabies virus in ChatCre/+; Rosa26RΦGT mice.

Figure 12—figure supplement 5.

Figure 12—figure supplement 5—source data 1. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from PL injection 170125n7 (UCL).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 5—source data 2. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from PL injection 170125n8 (UCL).
Figure 12—figure supplement 5—source data 3. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 170125n7 (UCL).
Figure 12—figure supplement 5—source data 4. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 170125n8 (UCL).
Figure 12—figure supplement 6. Distribution of motor neurons (triangles) and premotor interneurons (dots) of 4 MG (dark blue) single injections (UoG) of rabies virus in ChatCre/+; Rosa26RΦGT mice.

Figure 12—figure supplement 6.

Figure 12—figure supplement 6—source data 1. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from MG injection 1605 (UoG).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 6—source data 2. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from MG injection 1660 (UoG).
Figure 12—figure supplement 6—source data 3. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from MG injection 1661 (UoG).
Figure 12—figure supplement 6—source data 4. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from MG injection 1662 (UoG).
Figure 12—figure supplement 7. Distribution of motor neurons (triangles) and premotor interneurons (dots) of 3 PL (dark orange) single injections (UoG) of rabies virus in ChatCre/+; Rosa26RΦGT mice.

Figure 12—figure supplement 7.

Figure 12—figure supplement 7—source data 1. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from PL injection 1611 (UoG).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 7—source data 2. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from PL injection 1613 (UoG).
Figure 12—figure supplement 7—source data 3. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from PL injection 1640 (UoG).
Figure 12—figure supplement 8. Distribution of motor neurons (triangles) and premotor interneurons (dots) of 3 LG (blue) and TA (yellow) double injections with low titre rabies virus in ChatCre/+; Rosa26RΦGT mice (UoG).

Figure 12—figure supplement 8.

Figure 12—figure supplement 8—source data 1. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 1644 (UoG).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 8—source data 2. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 1646 (UoG).
Figure 12—figure supplement 8—source data 3. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 1653 (UoG).
Figure 12—figure supplement 8—source data 4. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 1644 (UoG).
Figure 12—figure supplement 8—source data 5. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 1646 (UoG).
Figure 12—figure supplement 8—source data 6. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 1653 (UoG).
Figure 12—figure supplement 9. Distribution of motor neurons (triangles) and premotor interneurons (dots) of 4 LG (blue) single injections with low titre rabies virus in ChatCre/+; Rosa26RΦGT mice (UoG).

Figure 12—figure supplement 9.

Figure 12—figure supplement 9—source data 1. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 1656 (UoG).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 9—source data 2. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 1657 (UoG).
Figure 12—figure supplement 9—source data 3. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 1570 (UoG).
Figure 12—figure supplement 9—source data 4. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from LG injection 1571 (UoG).
Figure 12—figure supplement 10. Distribution of motor neurons (triangles) and premotor interneurons (dots) of 3 TA (yellow) single injections with low titre rabies virus in ChatCre/+; Rosa26RΦGT mice (UoG).

Figure 12—figure supplement 10.

Figure 12—figure supplement 10—source data 1. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 1573 (UoG).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 10—source data 2. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 1574 (UoG).
Figure 12—figure supplement 10—source data 3. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 1639 (UoG).
Figure 12—figure supplement 11. Distribution of motor neurons (triangles) and premotor interneurons (dots) of 4 GS (blue) single injections with rabies virus in Olig2Cre/+; Rosa26RΦGT mice (MDC).

Figure 12—figure supplement 11.

Figure 12—figure supplement 11—source data 1. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from GS injection 353 (MDC).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 11—source data 2. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from GS injection 399 (MDC).
Figure 12—figure supplement 11—source data 3. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from GS injection 1332 (MDC).
Figure 12—figure supplement 11—source data 4. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from GS injection 1349 (MDC).
Figure 12—figure supplement 12. Distribution of motor neurons (triangles) and premotor interneurons (dots) of 3 TA (yellow) single injections with rabies virus in Olig2Cre/+; Rosa26RΦGT mice (MDC).

Figure 12—figure supplement 12.

Figure 12—figure supplement 12—source data 1. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 700 (MDC).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 12—source data 2. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 721 (MDC).
Figure 12—figure supplement 12—source data 3. Cartesian x-y-z coordinates of interneurons (sheet 1) and motor neurons (sheet 2) from TA injection 1324 (MDC).
Figure 12—figure supplement 13. Distribution of infected neurons (primary infected motor neurons or secondary infected interneurons are not distinguished) of 2 GS (blue) and TA (yellow) double injections and 2 GS single injections of rabies and AAV-Ef1a-B19G in wild-type mice (Salk).

Figure 12—figure supplement 13.

Figure 12—figure supplement 13—source data 1. Cartesian x-y-z coordinates of infected neurons from GS injection 1 (Salk).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 13—source data 2. Cartesian x-y-z coordinates of infected neurons from GS injection 2 (Salk).
Figure 12—figure supplement 13—source data 3. Cartesian x-y-z coordinates of infected neurons from GS injection a (Salk).
Figure 12—figure supplement 13—source data 4. Cartesian x-y-z coordinates of infected neurons from GS injection b (Salk).
Figure 12—figure supplement 13—source data 5. Cartesian x-y-z coordinates of infected neurons from TA injection 1 (Salk).
Figure 12—figure supplement 13—source data 6. Cartesian x-y-z coordinates of infected neurons from TA injection 2 (Salk).
Figure 12—figure supplement 14. Distribution of infected neurons (primary infected motor neurons or secondary infected interneurons are not distinguished) of 4 GS (blue) single injections of rabies and AAV-CAG-Flex-oG in ChatCre/+ mice (Salk).

Figure 12—figure supplement 14.

Figure 12—figure supplement 14—source data 1. Cartesian x-y-z coordinates of infected neurons from GS injection 22a_4 (Salk).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 14—source data 2. Cartesian x-y-z coordinates of infected neurons from GS injection 26a_1 (Salk).
Figure 12—figure supplement 14—source data 3. Cartesian x-y-z coordinates of infected neurons from GS injection 26a_2 (Salk).
Figure 12—figure supplement 14—source data 4. Cartesian x-y-z coordinates of infected neurons from GS injection 26a_4 (Salk).
Figure 12—figure supplement 15. Distribution of infected neurons (primary infected motor neurons or secondary infected interneurons are not distinguished) of 3 TA (yellow) single injections of rabies and AAV-CAG-Flex-oG in ChatCre/+ mice (Salk).

Figure 12—figure supplement 15.

Figure 12—figure supplement 15—source data 1. Cartesian x-y-z coordinates of infected neurons from TA injection 26_1 (Salk).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 15—source data 2. Cartesian x-y-z coordinates of infected neurons from TA injection 26_3 (Salk).
Figure 12—figure supplement 15—source data 3. Cartesian x-y-z coordinates of infected neurons from TA injection 26_4 (Salk).
Figure 12—figure supplement 16. Distribution of infected neurons (primary infected motor neurons or secondary infected interneurons are not distinguished) of 4 GS (blue) and TA (yellow) double injections of PRV-152 and PRV-614 in wild type mice (Salk).

Figure 12—figure supplement 16.

Figure 12—figure supplement 16—source data 1. Cartesian x-y-z coordinates of infected neurons from GS injection 1_1 (Salk).
The x-y coordinates are normalized (between –0.5 and 0.5) and scaled to an idealized spinal cord of dimensions 1700 μm and 900 μm in the mediolateral and dorsoventral directions respectively, while z coordinates are in micron, with the axis origin taken at the border between L4 and L5 and negative values in the rostral direction.
Figure 12—figure supplement 16—source data 2. Cartesian x-y-z coordinates of infected neurons from GS injection 1_4 (Salk).
Figure 12—figure supplement 16—source data 3. Cartesian x-y-z coordinates of infected neurons from GS injection 2_2 (Salk).
Figure 12—figure supplement 16—source data 4. Cartesian x-y-z coordinates of infected neurons from GS injection 3_3 (Salk).
Figure 12—figure supplement 16—source data 5. Cartesian x-y-z coordinates of infected neurons from TA injection 1_1 (Salk).
Figure 12—figure supplement 16—source data 6. Cartesian x-y-z coordinates of infected neurons from TA injection 1_4 (Salk).
Figure 12—figure supplement 16—source data 7. Cartesian x-y-z coordinates of infected neurons from TA injection 2_2 (Salk).
Figure 12—figure supplement 16—source data 8. Cartesian x-y-z coordinates of infected neurons from TA injection 3_3 (Salk).