Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Rabies virus) | ΔG-Rab-eGFP | Gift from M. Tripodi lab, LMCB Cambridge | ||
| Strain, strain background (Rabies virus) | ΔG-Rab-mCherry | Gift from M. Tripodi lab, LMCB Cambridge | ||
| Strain, strain background (Adeno associated virus) | AAV6-Ef1a-B19G | Produced by Applied Viromics (USA) | ||
| Strain, strain background (Adeno associated virus) | AAV6-CAG-Flex-oG | Produced at the Salk GT3 virus core facility | ||
| Strain, strain background (M. musculus, ChatCre/+) | ChAT-IRES-Cre | Jackson laboratory | IMSR Cat# JAX:006410; RRID:IMSR_JAX:006410 | allele symbol: Chattm2(cre)Lowl; maintained on a C57BL6/J background |
| Strain, strain background (M. musculus, Olig2Cre/+) | Olig2-Cre | Jackson laboratory | IMSR Cat# JAX:025567; RRID:IMSR_JAX:025567 | allele symbol: B6.129-Olig2tm1.1(cre)Wdr/J maintained on a C57BL6/J background |
| Strain, strain background (M. musculus, Rosa26RΦGT) | RΦGT | Jackson laboratory | IMSR Cat# JAX:024708; RRID:IMSR_JAX:024708 | allele symbol: Gt(ROSA)26Sortm1(CAG-RABVgp4,-TVA)Arenk; maintained on a C57BL6/J background |
| Strain, strain background (M. musculus, Slc6A5eGFP) | Slc6A5eGFP | Gift from H. Zeilhofer lab, University of Zurich | IMSR Cat# RBRC04708; RRID:IMSR_RBRC04708 | allele symbol: Tg(Slc6a5-EGFP)1Uze; maintained on a C57BL6/J background |
| Strain, strain background (M. musculus, Rosa26RCL-tdTom) | Ai9(RCL-tdT) | Jackson laboratory | IMSR Cat# JAX:007909; RRID:IMSR_JAX:007909 | allele symbol: Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J; maintained on a C57BL6/J background |
| Cell line (Homo-sapiens, female) | HEK293t/17 | Gift from M. Tripodi lab, LMCB Cambridge | RRID:CVCL_1926 | ATCC, cat. no. CRL-1126 |
| Cell line (Mesocricetus auratus, male) | BHK-21 | Gift from M. Tripodi lab, LMCB Cambridge | RRID: CVCL_1915 | ATCC # CCL-10 |
| Cell line (Mesocricetus auratus, male) | BHK-G | Gift from M. Tripodi lab, LMCB Cambridge | RRID:CVCL_1915 | Derived from ATCC # CCL-10 |
| Antibody (UCL) |
Anti-ChAT (Goat polyclonal) | Millipore | Cat# AB144P; RRID:AB_2079751 | IF (1:100) |
| Antibody (UCL) |
Anti-mCherry (Chicken polyclonal) | Abcam | Cat# ab205402; RRID:AB_2722769 | IF (1:2500) |
| Antibody (UCL) |
Anti-GFP (Rabbit polyclonal) | Abcam | Cat# ab290; RRID:AB_303395 | IF (1:2500) |
| Antibody (UCL) |
Anti-vGluT2 (Guinea pig polyclonal) | Millipore | Cat# AB2251-I; RRID:AB_2665454 | IF (1:2500) |
| Antibody (UCL) |
Anti-Isl1 (Guinea pig polyclonal) | Gift from T. Jessell lab, Columbia University, New York | IF (1:7500) | |
| Antibody (UCL) |
Anti-guinea pig IgG H&L Alexa Fluor 647 (Donkey polyclonal) | Millipore | Cat# AP193SA6; RRID:AB_2340477 | IF (1:700) |
| Antibody (UCL) |
Anti-Goat IgG H&L Alexa Fluor 405 (Donkey polyclonal preadsorbed) | Abcam | Abcam Cat# AB175665; RRID:AB_2636888 | IF (1:200) |
| Antibody (UCL) |
Anti-Rabbit IgG H&L Alexa Fluor488 (Donkey polyclonal Highly Cross-Adsorbed) | Thermo Fisher Scientific | Cat# A-21206; RRID:AB_2535792 | IF (1:1000) |
| Antibody (UCL) |
Anti-Chicken IgY (IgG) H&L Cy3-AffiniPure (Donkey polyclonal) | Jackson ImmunoResearch Labs | Cat# 703-165-155; RRID:AB_2340363 | IF (1:1000) |
| Antibody (Glasgow University) | Anti-GFP (chicken polyclonal) | Abcam | Cat# Ab13970 RRID:AB_300798 |
IF (1:1000) |
| Antibody (Glasgow University) | Anti-mCherry (rabbit polyclonal) | Abcam | Cat# Ab167453 RRID:AB_2571870 |
IF (1:2000) |
| Antibody (Glasgow University) | Anti-chicken IgY H&L Alexa Fluor488 (Donkey polyclonal) | Jackson ImmunoResearch Labs | Cat# 703-545-155; RRID:AB_2340363 | IF (1:500) |
| Antibody (MDC) | Anti-ChAT (rabbit polyclonal) | Abcam | Cat# Ab2750952 RRID:AB_2750952 |
IF (1:16,000) |
| Antibody (MDC) | Anti-Rabbit IgG H&L Alexa Fluor488 (Donkey polyclonal) | Thermo Fisher Scientific | Cat# A-21206; RRID:AB_2535792 | IF (1:1000) |
| Antibody (Salk) | Anti-GFP (goat polyclonal) | Rockland | Cat#600-101-215; RRID:AB_218182 | IF (1:1000) |
| Antibody (Salk) | Anti-RFP (rabbit polyclonal) | Rockland | Cat#600-401-379; RRID:AB_2209751 | IF (1:1000) |
| Antibody (Salk) | Anti-goat IgY H&L Alexa Fluor488 (Donkey polyclonal) | Invitrogen | Cat#A11055; RRID:AB_2534102 | IF (1:1000) |
| Antibody (Salk) | Anti-rabbit IgY H&L Alexa Fluor555 (Donkey polyclonal) | Invitrogen | Cat#A32794; RRID:AB_2762834 | IF (1:1000) |
| Chemical compound, drug | Mowiol 4–88 | Sigma Aldrich | Cat# 81381–250 G | |
| Software, algorithm | ZEN Digital Imaging for Light Microscopy: Zen Blue 2.3 | Carl Zeiss light microscopy imaging systems | RRID:SCR_013672 | |
| Software, algorithm | Imaris 9.1 | Bitplane | RRID:SCR_007370 | |
| Software, algorithm | Adobe illustrator version CC 2019 |
Adobe | RRID:SCR_010279 | |
| Software, algorithm | Matlab version 2021b | Mathworks | RRID:SCR_001622 |