Extended Data Table 2.
Cryo-EM data collection and processing statistics of FTLD-TDP donors 2–4.
| Donor 2 | Donor 3 | Donor 4 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| PM1 | PM2 | PM3 | PM1 | PM2 | PM3 | PM1 | PM2 | PM3 | |
| Magnification | ×81,000 | ×81,000 | ×81,000 | ×81,000 | ×81,000 | ×81,000 | ×81,000 | ×81,000 | ×81,000 |
| Voltage (kV) | 300 | 300 | 300 | 300 | 300 | 300 | 300 | 300 | 300 |
| Electron exposure (e–/Å2) | 37 | 37 | 37 | 36 | 36 | 36 | 36 | 36 | 36 |
| Defocus range (μm) | 1.0–5.1 | 1.0–5.1 | 1.0–5.1 | 0.5–5.1 | 0.5–5.1 | 0.5–5.1 | 0.8–4.9 | 0.8–4.9 | 0.8–4.9 |
| Pixel size (Å) | 1.1 | 1.1 | 1.1 | 1.1 | 1.1 | 1.1 | 1.1 | 1.1 | 1.1 |
| Symmetry imposed | C1 | C2 | C1 | C1 | C2 | C1 | C1 | C2 | C1 |
| Helical rise (Å) | 4.896 | 4.9 | 2.446 | 4.897 | 4.906 | 2.452 | 4.897 | 4.904 | 2.4281 |
| Helical twist (°) | −0.413 | 179.619 | 179.807 | −0.413 | 179.6 | 179.8 | −0.413 | 179.605 | 179.81 |
| Initial particle images | 438,393 | 438,393 | 438,393 | 419,107 | 419,107 | 419,107 | 228,432 | 228,432 | 228,432 |
| Final particle images | 38,894 | 24,420 | 5,317 | 26,533 | 15,587 | 5,335 | 14,407 | 13,482 | 5,798 |
| Map resolution (Å) | 3.8 | 4.0 | 4.6 | 3.6 | 4.1 | 5.3 | 3.5 | 3.6 | 5.0 |
| FSC threshold | 0.143 | 0.143 | 0.143 | 0.143 | 0.143 | 0.143 | 0.143 | 0.143 | 0.143 |
| Map resolution range (Å) | 200–3.8 | 200–4.0 | 200–4.6 | 200–3.6 | 200–4.1 | 200–5.3 | 200–3.5 | 200–3.6 | 200–5.0 |