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. 2023 Jan 17;13(1):e1181. doi: 10.1002/ctm2.1181

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© 2023 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Schematics of scRNA‐seq and major cell types in ESCC. (A) Schematic illustration of the experimental workflow in our study. (B) UMAP plot showed 24 clusters from eight resected tumour tissues, two tumour biopsies and seven normal tissues. (C) The UMAP plots were coloured by sample origin. (D) UMAP plot showed seven major cell types using canonical marker genes. (E) UMAP plots showed the expression levels of canonical marker genes. (F) Heatmap of the canonical marker genes in each major cell type. (G) The number of the major cell types in the sample origins.