Figure 2: X-gal staining temperature and buffer optimization experiment.
Optimal staining protocol was a 1:250 x-gal dilution in 1X PBS at 22°C. A) X-gal staining in 1X PBS or Z-buffer at 4°C. B) X-gal staining in 1X PBS or Z-buffer at 22°C. C) X-gal staining in 1X PBS or Z-buffer at 37°C. Scale bar = 1 mm.