Figure 5. Impact of toxic challenge with ddC on CB2, cytokine and chemokine mRNA expression levels in lumbar spinal cord derived from male wildtype mice.
ddC treatment produced an upregulation of IL-1β, TNF-α, and CCL2 in the lumbar spinal cord compared with the saline group similarly injected with vehicle (i.p.). Values were calculated using the 2(−ΔΔCt) method. GAPDH was used as reference gene. Data are expressed as mean ± S.E.M. (n=4 per group). * p<0.05 vs. vehicle, unpaired sample two tailed t-test.