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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Pharmacol Res. 2022 Nov 20;187:106560. doi: 10.1016/j.phrs.2022.106560

Figure 6. Impact of ddC and systemic (i.p.) administration of AM1710, morphine and LY2828360 on expression levels of IL-1β, TNF-α and CCL2 mRNAs in lumbar spinal cord derived from male wildtype mice.

Figure 6.

(A, B, C) AM1710 blunts ddC-evoked increases in IL-1β, TNF-α and CCL2 mRNAs, morphine blunts ddC-induced increases in IL-1β, TNF-α mRNA’s (Mor; D,E,F), whereas LY2828360 fails to alter ddC-induced increases in IL-1β, TNF-α and CCL2 mRNAs (LY; G, H, I). ddC treatment (Veh ddC) induced upregulation of IL-1β, TNF-α and CCL2 mRNAs compared to saline treated groups that were similarly injected with vehicle (Veh-Veh). Values were calculated using the 2(−ΔΔCt) method with GAPDH was used as reference gene and all groups compared to the Veh Veh group. Data are expressed as mean ± S.E.M. (n=4 per group). * p<0.05, one-way ANOVA.