Figure 1. Schematic diagram of the BATCH screening workflow.

The BATCH screening involves sgRNA variants mediated multilevel CRISPRi of genes of interest within metabolic network to redirect carbon flux to target biosynthetic pathways. Improved production phenotypes will be reflected by the target chemical inducible reporter GFP fluorescence. The BATCH screening consists of a first round plate based selection and a second round test tube based selection followed by final shake flask based validation. Blue lines indicate biosynthetic pathways and red lines indicate relevant or potential competing pathways.