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. 2023 Jan 17;14:276. doi: 10.1038/s41467-023-35876-8

Fig. 4. Mitochondrial disruption after irradiation with a UV-nail dryer in HEKa cells.

Fig. 4

a Evaluating the mitochondrial membrane potential after irradiation of HEKa cells with a UV-nail dryer. DAPI is shown in blue, MitoTracker in green, and tetramethylrhodamine dye (TMRM) in red. Yellow corresponds to overlaps between MitoTracker and TMRM. Images collected from three independent experiments. b Examining mitochondrial ROS production in HEKa cells. DAPI is shown in blue, MitoTracker in green, and MitoSOX in red. Yellow corresponds to overlaps between MitoTracker and MitoSOX. In both (a) and (b), MitoTracker green reagent is used to localize mitochondria regardless of mitochondria membrane potential. Images collected from three independent experiments. c Quantification of the number of MitoSOX foci in 100 cells per condition in HEKa, examined over 4 independent replicates. The bounds of the boxplots represent the interquartile range divided by the median, and Tukey-style whiskers extend to a maximum of 1.5 × interquartile range beyond the box. Statistically significant results from FDR corrected Mann–Whitney U two-sided tests are denoted as q-values.