Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 17;14:267. doi: 10.1038/s41467-023-35910-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Crown 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Distal ATAC sites containing at least one fragment scoring positive in our assay. Source data are provided as a Source Data file. b Percentage of functionally identified distal elements scoring positive in our screen overlapping with histone H3 lysine 27 (H3K27Ac) peaks as compared to inactive elements or where activity is not known. ChIP data from ref. ¹⁸. Source data are provided as a Source Data file. c Overlap of fragments scoring positive in our assay with known enhancers from the VISTA database²⁶. Source data are provided as a Source Data file. d Percentage intergenic and intragenic fragments scoring positive in our assay overlapping with RNA polymerase II binding sites in the hemogenic endothelium and HP cells. Pol II data from ref. ³⁰. Source data are provided as a Source Data file. e Presence or absence of enhancer activity at the different developmental stages expressed as binary code (0 = scoring negative; 1 = scoring positive) and sorted by the activity pattern across differentiation. The heatmap depicts the activity of the genes associated with these elements. Enhancer - promoter association was determined by using the union of HiC data from ES and HPC7 (HP) cells²⁹ together with co-regulation data determined by ref. ²¹ in a total of 21,671 elements. All promoters not covered by these data (5599) were associated by being the nearest to the enhancer element. f–h Activity profiles of individual enhancers identified in our screen in single ES cell clones during differentiation. The presence or absence of an ATAC-peak is indicated by the binary code used in (e). f RUNX1 + 3.7 enhancer element (chr16:92822182-92822601). g Dlk1 enhancer element (Chr12: 109437601-109438085), h RUNX1 + 23 kb enhancer element⁴⁹. Data are presented as mean values + /− standard deviation (SD). Dots showing individual values for Dlk1 and Runx1 + 3.7 kb n = 3 biologically independent experiments and for Runx1 + 23 kb n = 6 biologically independent experiments. For sequence details see Supplementary Notes. Source data are provided as a Source Data file.