Fig. 7. HE-specific and cytokine responsive gene expression is mediated by the interplay of TEAD, AP-1 and RUNX1.

a–c Activity of the Sparc, Pxn and Hspg2 enhancers (for details see Supplementary Notes) in isolation in the presence and absence of VEGF. Data are presented as mean values + /− SD. Dots showing individual values for n = 3 biologically independent experiments. P-values calculated using a two-sided Student’s t-test. Source data are provided as a Source Data file. d HE-specific expression of Galnt1 (data from ref. ¹⁸), reporter gene activity driven by wild type (WT) and mutated Galnt1 enhancer elements in the presence and absence of the indicated cytokines. Data are presented as mean values + /− SD. Dots showing individual values for n = 3 biologically independent experiments. e Sequence of the Galnt1 enhancer with TF binding motif mutations indicated in red. Strong consensus sequences are highlighted in red. Source data are provided as a Source Data file. f Chip data showing the binding of the indicated transcription factors to RUNX1 enhancers in HE and HP cells. g Effect of mutations of the TEAD and RUNX1 binding sites on the individual activity of the RUNX1 + 23 kb enhancer in serum culture. Data are presented as mean values + /− SD. Dots showing individual values for n = 6 biologically independent experiments. P-values calculated using a two-sided Student’s t-test. Source data are provided as a Source Data file.