FIG. 2.

Parenteral injection with Pw, but not with Pa, induces convulsive behavior in mice. BALB/c mice were injected with Pw, Pa, or PBS and 2 h later were placed at 37°C (Pw/37, Pa/37, or PBS/37). Alternatively, mice were left at room temperature after injection of Pw (Pw/rt). (A) Behavioral activity was recorded for a further hour. (B) Mice were sacrificed, and hippocampal IL-1β protein concentrations were determined by immunoassay. (C) Behavioral changes were also recorded after injection of B. pertussis LPS (6 μg), PT (0.1 μg), LPS and PT, Pw, or PBS and exposure to 37°C. Results are mean (SE) values for six mice in each test group. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001 Pw versus PBS controls.