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. Author manuscript; available in PMC: 2023 Oct 18.
Published in final edited form as: J Immunol. 2022 Aug 1;209(3):569–581. doi: 10.4049/jimmunol.2200065

Figure 4. IL-1β increases Icam1 gene promoter activity, mRNA content, and protein abundance in 832/13 cultured rat β-cells.

Figure 4.

A. 832/13 insulinoma cells were not treated (NT) or stimulated with 1 ng/mL IL-1β for the indicated times. B. 832/13 cells were either untreated (NT) or treated with the indicated concentrations of IL-1β for 3 h. C. 832/13 cells were treated with 1 ng/mL IL-1β, 100 U/mL IFN-γ, or a combination of both cytokines for 3 h. D. 832/13 cells were transfected with Icam1 promoter luciferase constructs containing either −1kb or −3kb of the Icam1 gene promoter. 24 h post-transfection, cells were either untreated or stimulated with 1 ng/mL IL-1β, 100 U/mL IFN-γ, or a combination of both cytokines for 4 h. Data are normalized to the respective NT control for each construct (−1kb and −3kb). E. Cells were untreated or treated with 1 ng/mL IL-1β or a combination of both IL-1β and 100 U/mL IFN-γ for either 4 h or 8 h. β-actin is shown as a control for protein loading. An image representative of two independent experiments is shown. ****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05. p values are vs. respective NT controls unless indicated otherwise in figures. ns = not significant.