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. 2022 Dec 19;10:rbac105. doi: 10.1093/rb/rbac105

Figure 5.

Figure 5.

Determine the anatomy of SGs and their surrounding microvasculature using tissue clearing technique and histological staining. (A) Schematic showing 3D imaging of the SG-vasculature anatomical structure by using the tissue-clearing technique. (B) Light-sheet microscopy images showing the close proximity of SGs and their vascular beds after optical clearing. Arrows and arrowheads indicate SGs and microvessels, respectively. Dashed boxes highlight the subepidermal ducts and nearby vascular arcades. (C) Conventional histological sections confirming the capillaries (CD31) closely surround the SGs (K18 and ATP1A1). CD31, red; K18, ATP1A1, green; DAPI, blue. Scale bars: (B) 100 μm; (C) 50 μm. Adapted with permission from Yuan et al. [167].