Table 3.
Application of in situ bioprinting in skin regeneration and wound repair
| Printing strategy | Scanning approach | Bioink | Laden cells | Models | Ref. |
|---|---|---|---|---|---|
| Automatic | |||||
| Extrusion-based bioprinting | NA | Fibrinogen + collagen | AFSCs, BMSCs | Murine full-thickness skin wound model | [118] |
| NA | Thiolated HA + thiolated gelatin + PEGDA | AFSCs | Murine full-thickness skin wound model | [119] | |
| (For soft tissue repair) droplet-based bioprinting | 3D laser scanner | (For soft tissue repair) fibrinogen + collagen + KGF | (For soft tissue repair) dermal fibroblasts | Murine composite skin and calvarial bone defect | [114] |
| Inkjet-based bioprinting | 3D laser scanner | Fibrinogen + collagen | Dermal fibroblasts, epidermal keratinocytes | Murine and porcine full-thickness skin wounds | [91] |
| Two-photon bioprinting | NA | HCC-PEG | Muscular fibroblasts | Printing inside murine normal dermis | [111] |
| Handheld | |||||
| Extrusion-based bioprinting | Not required | GelMA + VEGF | NA | Porcine full-thickness skin wound model | [109] |
| Fibrinogen + HA | UMSCs | Porcine full-thickness skin burn injury model | [116] | ||
| Alginate + collagen; fibrinogen + HA + collagen | (For in vitro experiments) dermal fibroblasts, epidermal keratinocytes |
Murine and porcine full-thickness skin wounds | [117] | ||
| Alginate + gelatin + PRP | Dermal fibroblasts, epidermal stem cells | Murine full-thickness skin wound model | [66] | ||
AFSCs, amniotic fluid-derived stem cells; BMSCs, bone marrow-derived mesenchymal stem cells; GelMA, gelatin methacryloyl; HA, hyaluronic acid; HCC, 7-hydroxycoumarin-3-carboxylate; KGF, keratinocyte growth factor; NA, not applicable; PEG, polyethylene glycol; PRP, platelet-rich plasma; UMSCs, umbilical cord-derived mesenchymal stromal cells; VEGF, vascular endothelial growth factor.