TABLE 1.
Binding affinities of all tested ligands to the 3 CSPs in E. onukii.
| Ligands | Source | CAS number | Purity (%) | EonuCSP4 | EonuCSP 6-1 | EonuCSP6-2 | |||
|---|---|---|---|---|---|---|---|---|---|
| IC50 (μM) | K i (μM) | IC50 (μM) | K i (μM) | IC50 (μM) | Ki (μM) | ||||
| Methyl Salicylate | TCI | 119-36-8 | >99% | U.d. | U.d. | U.d. | U.d. | U.d. | U.d. |
| Ocimene | MACKLIN | 13877-91-3 | ≥90% | 21.96 | 18.93 | U.d. | U.d. | U.d. | U.d. |
| Cis-3-Hexenyl n-valerate | TCI | 35852-46-1 | >98% | U.d. | U.d. | U.d. | U.d. | U.d. | U.d. |
| β-ionone | MACKLIN | 79-77-6 | 95% | U.d. | U.d. | U.d. | U.d. | U.d. | U.d. |
| Ethyl Benzoate | J&K | 93-89-0 | >99% | U.d. | U.d. | U.d. | U.d. | U.d. | U.d. |
| Benzaldehyde | MERCK | 100-52-7 | 99% | U.d. | U.d. | 21.32 | 18.54 | U.d. | U.d. |
| Cis-3-Hexrnyl Butyrate | MACKLIN | 16497-36-4 | ≥98% | U.d. | U.d. | U.d. | U.d. | U.d. | U.d. |
| (-)-Linalool | MACKLIN | 126-91-0 | ≥95% | U.d. | U.d. | U.d. | U.d. | U.d. | U.d. |
| Ethyl Decanoate | TCI | 110-38-3 | >98% | U.d. | U.d. | U.d. | U.d. | U.d. | U.d. |
| Cis-3-Hexenyl Hexanoate | MACKLIN | 31501-11-8 | 98% | U.d. | U.d. | U.d. | U.d. | U.d. | U.d. |
| Cis-3-Hexen-1-ol | ALADDIN | 928-96-1 | 98% | U.d. | U.d. | U.d. | U.d. | U.d. | U.d. |
| Limonene | MACKLIN | 5989-27-5 | >99% | U.d. | U.d. | U.d. | U.d. | U.d. | U.d. |
| Farnesene | ALADDIN | 502-64-4 | 98% | 20.17 | 17.85 | U.d. | U.d. | U.d. | U.d. |
| trans-β-Farnesene | ALADDIN | 18794-84-8 | 98% | U.d. | U.d. | U.d. | U.d. | U.d. | U.d. |
| 3-Carene | TCI | 13466-78-9 | 90% | U.d. | U.d. | U.d. | U.d. | U.d. | U.d. |
“U.d.” means that the K i exceeded 20 μM. Low K i values mean high binding affinity for protein and the test volatiles. Purity (%) showed the ligand concentration range.