Fig. 5.

Acute deletion of UBE4B in the forebrain induces KLHL22-dependent mTOR hyperactivation. (A,B) Ube4b sgRNAs (in the PX459 vector) were delivered with or without Klhl22 sgRNAs into the hippocampal region of E14.5 embryos by in utero electroporation (as illustrated in B). pCIG2-GFP was co-delivered to mark electroporated cells. Brains were collected at birth and pS6 signals were examined. Images of GFP⁺ cells in the DG were acquired at 10× (left) and 100× magnification (right), respectively. Arrows indicate cells with elevated pS6 signals. Dashed line delineates the DG region. Scale bars: 50 μm. (C,D) Ratio of pS6⁺ cells (C) and average intensity of pS6 signals (D) in GFP-expressing cells in electroporated DGs at P0. Three animals for each experimental group were quantified. Data represent mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001 (relative to indicated controls; one-way ANOVA).