Fig. 6.

Deletion of UBE4B in the brain impairs proliferation and differentiation of NPCs. (A-D) Immunohistochemistry of SOX2 and Ki67 in the DG at P0 (A). Scale bar: 50 μm. Three pairs of P0 Ube4b^f/f and Ube4b Nestin-CKO littermates were analyzed for the number of SOX2⁺ NPCs (B), the number of Ki67⁺ proliferating cells (C) and the percentage of proliferating NPCs (SOX2⁺ Ki67⁺) (D) in the DG. Data represent mean±s.e.m. *P<0.05, **P<0.01 (relative to Ube4b^f/f; unpaired, two-tailed Student's t-test). (E-G) Reduction of proliferating and total PAX6⁺ NPCs in the DG of Ube4b Nestin-CKO. Scale bar: 50 μm. A single pulse of BrdU was injected into pregnant dams at E19.5 to label proliferating cells. Injected mice were sacrificed 12 h later. Genotype-confirmed Ube4b^f/f and Ube4b Nestin-CKO pups were processed for BrdU and PAX6 staining. BrdU⁺ PAX6⁺ cells were considered proliferating NPCs. Brain slices from three pairs of Ube4b^f/f and CKO pups were counted and analyzed (F,G). Data represent mean±s.e.m. *P<0.05, **P<0.01 (relative to Ube4b^f/f; unpaired, two-tailed Student's t-test). (H,I) Immunohistochemistry (H) and quantification (I) of CTIP2⁺ GCs in the DG at the indicated postnatal stages. Scale bars: 50 μm. n=3, 3, 4 and 3 pairs of Ube4b^f/f and CKO littermates for P0, P7, P14 and P30, respectively. Data represent mean±s.e.m. *P<0.05 (relative to Ube4b^f/f at the same age; unpaired, two-tailed Student's t-test).