Fig. 6.

IMC10 knockdown exhibit other mitochondrial distribution and division phenotypes. IFA of knockdown parasites stained for IMC3 or acetylated tubulin (cyan) and F₁B-ATPase (magenta) showed aberrant phenotypes. (A) Broken lasso. (B) Amitochondriate parasites. (C) Accumulation of mitochondrion material outside of the cells within the same vacuole. (D,E) IFA of knockdown parasites stained for IMC6 (cyan) and F₁B-ATPase (magenta) showing aberrant phenotypes. Arrows in A–C highlight, respectively, a broken mitochondrion, a amitochondriate parasite and mitochondrial material accumulated outside of the parasites. For A, B, and C, images were acquired 24 h post treatment. In the graph, the left two columns are 24 h post treatment, and right 48 h post treatment. −, without ATc, + with ATc. (D) Aberrant number of budding cells within the same mother. Arrowheads indicate three daughter cells budding inside of a single mother. (E) Asynchronous vacuoles in which parasites are in different stages of division. Arrowheads indicate two different phenotypes within the same vacuole, abnormal daughter cell number and lack of synchronicity. For both D and E, parasites were inspected after 48 h in the presence of ATc. All graphs represent the mean±s.d. percentage of vacuoles with the related phenotype. At least 150 vacuoles per sample were inspected. For all graphs, n=3. Each replicate is represented by a different color. Images were acquired using a Nikon 80i Eclipse microscope. Scale bars: 5 µm.