Fig. 7.

LMF1 and IMC10 interact during mitochondrial distribution. (A) U-ExM of intracellular parasites stained for LMF1-HA (yellow) and IMC10-Myc (magenta). On the left is the fluorescence signal showing the distribution of both proteins in the expanded parasites, followed by an overlay of that image with the signal for NHS-ester. On the right is a representation of the observed pattern of localization. See Movies 2–5 for the full Z-stack view. (B) Left, detail of two late-stage division cells with an emerging mitochondrion branch. Right, scheme depicting the relative localization of the proteins, including structures such as the basal complex, mitochondrion, LMF1 (yellow circles), and pellicle. Images were acquired using a Zeiss LSM 800 microscope with Airyscan processing. Scale bars: 5 μm. Results shown are representative of three repeats.