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. 2023 Jan 4;10:1058846. doi: 10.3389/fcell.2022.1058846

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Copyright © 2023 Asal, Koçak, Sarı, Reçber, Nemutlu, Utine and Güven.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of early lacrimal gland development markers in the protein and mRNA level on day 21 of differentiation confirms emergence of lacrimal gland stem and progenitor cells, (A) KRT14+/PAX6+/P63 + lacrimal gland multipotent epithelial stem cells, (B) KRT13+/P63 + lacrimal gland progenitor cells, (C) FOXC1+/PITX2+ periocular mesenchymal cells (Figures are representative of three independent differentiation experiments, scale bar: 50 µm), (D) Flow cytometry analysis of organoids with epithelial (EpCAM) and neural crest (P75) markers. N = 2 biological replicates. (E) Gene expression profile of organoids (F) miRNA 205 PCR expression and FGF10 levels during the same time course. (One-Way ANOVA. Error bars represent standard error of mean. *p ≤ .05, n = 3 biological replicates).