| Experimental models: Cell lines |
|
| Mouse KPC pancreatic cancer cells (fresh aliquot of cells with a maximum of 3 passages were used per experiment). |
A/Prof Marina Pajic, The Garvan Institute of Medical Research, Australia. |
PMID: 28381539
|
|
| Experimental models: Organisms/strains |
|
| Mouse: C57BL/6 mice of either sex at 10 weeks of age. No sex differences were observed. |
In-house (Prof Matthias Ernst), The Olivia Newton-John Cancer Research Institute |
N/A |
|
| Other |
|
| DMEM/F-12 media |
Gibco |
Cat#11320033 |
| Dulbecco’s phosphate-buffered saline (PBS) |
Gibco |
Cat#14190144 |
| TrypLE™ Express |
Gibco |
Cat#12604-021 |
| 175 cm2 Rectangular Straight Neck Cell Culture Flask with Vented Cap |
Falcon |
Cat#353112 |
| Countess® Automated Cell Counter |
Thermo Fisher |
Cat#AMQAF2000 |
| Cell Counting Slides |
Bio-Rad |
Cat#1450011 |
| Trypan Blue Stain (0.4%) for use with the Countess™ Automated Cell Counter |
ThermoFisher |
Cat#T10282
|
| Isoflurane |
Baxter |
N/A |
| Carprofen |
Zoetis |
N/A |
| Bupivacaine |
AstraZeneca |
N/A |
| Polyvisc eye ointment |
Alcon |
N/A |
| 0.9% Saline solution |
Pfizer |
N/A |
| Electrical heat pads |
Pet stores |
N/A |
| Sterile drape |
Abena |
Cat#4115 |
| Anesthesia chamber/tubing/nose cone |
In-house (Austin Health Bioresources Facility) |
N/A |
| Forceps |
Braun |
Cat#BD952R |
| Scissors |
Braun |
Cat#BC106R |
| Hemostat |
Able Scientific |
Cat#AS13008-12 |
| 27G × 1/2″ hypodermic needles |
Terumo |
Cat#NN+2713R |
| 100 μL Hamilton syringe |
Sigma-Aldrich |
Cat#20702 |
| 1 mL insulin syringe |
Terumo |
Cat#SS∗05M2713KA |
| Gauze |
Multigate |
Cat#11-559B |
| Reusable cautery kit (includes cauterizer and 10 mm cautery tips) |
Able Scientific |
Cat#ASCCK-1700 |
| 5-0 coated Vicryl sutures (absorbable) |
Ethicon |
Cat#J433H |
| Wound stapler and remover (BD Autoclip™ Wound Closing System) |
Fisher Scientific |
Cat#22-275998 |
| Wound staples |
Fisher Scientific |
Cat#01-804-5 |