TABLE 1.
Effect of adhesion and opsonization on uptake of B. suis by freshly isolated human monocytes from three blood donors
| Parameter | Monocytes | Relative infection index or phagocytosis index after incubation in RPMI medium with:
|
SD | ςxb | P adherencec | |||
|---|---|---|---|---|---|---|---|---|
| RPMI | hiFCS | Fresh FCS | hiHISa | |||||
| Infection | Nonadherent | 86 | 85 | 86 | 86 | 4.47 | 1.58 | 0.0014 |
| Adherent | 18 | 28 | 32 | 38 | 26.16 | 9.25 | ||
| SD | 40.50 | 35.45 | 38.97 | 38.51 | ||||
| ςx | 20.25 | 17.73 | 19.49 | 19.26 | ||||
| P opsonizationc | 0.9754 | |||||||
| Phagocytosis | Nonadherent | 14.3 | 14.7 | 15.9 | 24.7 | 1.76 | 0.63 | 0.0004 |
| Adherent | 1.1 | 1.8 | 2.3 | 3.4 | 8.96 | 3.17 | ||
| SD | 7.85 | 7.67 | 8.45 | 17.25 | ||||
| ςx | 3.93 | 3.83 | 4.22 | 8.62 | ||||
| P opsonization | 0.1669 | |||||||
a
HIS, human Brucella-specific antiserum.
b
ςx, standard error.
c
P, level of significance based on two-way analysis of variance.