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. 2023 Jan 4;16:1077071. doi: 10.3389/fncel.2022.1077071

FIGURE 2.

FIGURE 2

Schwann cells-derived exosomes enhance the angiogenic activities of Endothelial Cells. (A) Immunofluorescence images of PKH67-labeled exosomes taken up by bEnd.3 cells. Scale bar = 50 μm. (B) The proliferation of bEnd.3 cells was performed by the EdU test. (C) Quantitative analysis of EdU-positive cells in each group. (D–F) Tube formation and migration were measured by the capillary network formation assay and transwell chamber migration assay 6 h after seeding bEnd.3 cells pretreated with PBS, or SCs-Exos. Photomicrographs of tube-like structures and quantification of the tube number. (G) Photomicrographs of bEnd.3 cells that migrated through the Transwell® membrane. The bEnd.3 cells were stained with crystal violet. (H) Quantification of the number of the migrated bEnd.3 cells. The data are presented as mean ± SD. **p < 0.01 SCs-Exos vs. Control group. N = 5 in each group. Scale bar = 500 μm.