FIGURE 4.

Integrin-β1 is required in mediating the role of SCs-Exos for promoting angiogenesis on Endothelial Cells. (A) The proliferation of bEnd.3 cells was performed by EdU test. (D) Quantitative analysis of EdU-positive cells in each group. (B,E,F) Tube formation and migration were measured by the capillary network formation assay and transwell chamber migration assay 6 h after seeding bEnd.3 cells pretreated with PBS, SCs^ctrlshRNA-Exos, or SCs^{sh Integrin–β1}-Exos. (C) Photomicrographs of tube-like structures and quantification of the tube number. Photomicrographs of bEnd.3 cells that migrated through the Transwell^® membrane. The bEnd.3 cells were stained with crystal violet. (G) Quantification of the number of the migrated bEnd.3 cells. The data are presented as mean ± SD. **p < 0.01 Control group vs. SCs^ctrlshRNA-Exos group, ^##p < 0.01 SCs^ctrlshRNA-Exos group vs. SCs^{shIntegrin–β1}-Exos group. N = 5 in each group. Scale bar = 500 μm.