Figure 1.

Determination of cellular cytotoxicity of phenolic preservatives and insulin fibrils in murine cell lines and primary cultures in vitro. To determine the in vitro cytotoxicity of phenolic compounds and insulin fibrils in murine cell lines, as well as primary cultures in vitro MTT cytotoxicity assays were used after 3 days of co-culture of cells and test agents. MTT assay was used for 3T3-L1 fibroblast cells (A), 3T3-Fat induced cells (B), raw macrophage (C), following 3-day in vitro incubation with agents. Each panel shows cells treated with diluent (grey) diluent + fibril (light blue), saline + fibril (dark blue), or insulin (red) at increasing concentrations. Cell viability is expressed as percent to saline control. MTT assay (panels A-C) data reflect absorbance at 570 nm. Error bars represent standard deviation. The concentration of all test reagents is based on dilutions in media. Full statistical analysis is presented in supplemental section Table S1.