TABLE 3.

Putative and known CWPs in the complete genome of S. pyogenes

Identification	Size (aa)a	Repeats	COOH terminus	Reference or comment(s)b
Previously identified proteins
M1	484	Yes	LPSTGETANPFFTAAALTVMATAGVAAVVKRKEEN	21
GRAB	217	Yes	LPTTGEESNPFFTAAALAIMVSTGVLVVSSKCKEN	45
OrfX	379	Yes	LPSTGEKAQPLFIATMTLMSLFGSLLVTKRQKETKK	39
C5a peptidase	1,175	Yes	LPTTNDKDTNRLHLLKLVMTTFFLGLVAHIFKTKRTED	11
Serine protease	1,647	No	LPSTGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD	28
SclA	348	Yes	LPATGEQANPFFTAAAVAVMTTAGVLAVTKRKENN	44, 30
SclB	228	Yes	LPATGETTNPFFTAAAVAIMTTAGVVAVAKRQENN	31, 59; M. Rasmussen and L. Björck, submitted for publication
Proteins with suggested function
SntA	670	No	LPITGDNYKMSPIMTILALISLGGLNAFIKKRKS	Probable 5′ nucleotidases in Deinococcus radiodurans, E = 2 × 10−57
SpuA	1,165	No	LPKTGEASSKGLLAAGIALLLLAISLLMKRQKD	Alkaline amylopullulanase in Bacillus sp. E = 0 (D78258)
Proteins with unknown function
Cwp1	313	No	LPQTGERFSYYPVLLGLMILGLTPIMIPKKINN	No significant match
Cwp2	910	No	LPKTGDSLVYVITLLGTASLLVPILLLTKGKKES	(i) Probable secreted protein in Streptomyces coelicolor, E = 8 × 10−86 (T36922); (ii) Hypothetical protein in Treponema pallidum, E = 2 × 10−68 (AE001229)
Cwp3	215	No	LPSTGEMVSYVSALGIVLVATITLYSIYKKLKTSK	Unknown protein in S. pyogenes, E = 3 × 10−19 (U49397)
Cwp4	1,008	No	LPRTGSKGSFVYGILGYTSVALLSLITAIKKKKY	(i) Surface antigen BspA in Bacteroides forsythus, E = 4 × 10−21 (T31094); (ii) PcpA in S. pneumoniae, E = 3 × 10−7 (Z82001)
Cwp5	2,059	Yes	LPKSGDANSIVLVGLGVMSLLLGMVLYSKKKESKD	(i) Extracellular matrix binding protein in Abiotrophia defectiva, E = 2 × 10−61 (T31110); (ii) EF protein in S. suis, E = 5 × 10−49 (S33441)

^aaa, amino acids. 

^bFor previously identified proteins, relevant references are given. Previously unknown proteins were used as queries in a BLAST search of the GenBank database. The best hits are listed, with accession numbers and expect (E) values.