Figure 2.

miRNAs differentially expressed in serum of mice carrying orthotopic xenograft tumours of human SH-SY5Y cells. (A) miRNA expression levels in the serum of mice 6 days before and 10 days after engraftment with 10⁶ human SH-SY5Y cells. 94 miRNAs (in orange) were found differentially expressed between the two conditions (P < 0.05 and fold change (FC) >2, Wald test, corrected for multiple testing using Benjamin–Hochberg). (B) miRNA expression levels in the serum of mice 11 days after engraftment with 10⁶ human SH-SY5Y cells and non-engrafted, control mice. 61 miRNAs (in orange) were found differentially expressed between the two conditions (P < 0.05 and FC > 2, Wald test, corrected for multiple testing using Benjamin–Hochberg). (C) Overlap between the two groups of differentially expressed miRNAs. 57 miRNAs are differentially expressed in both comparisons. (D) Heatmap showing an increasing log2 fold change in serum miRNA expression between mice carrying orthotopic xenografts and non-engrafted mice over time. Time points: −6 = 6 days before engraftment; 11 = 11 days after engraftment; 15 = 15 days after engraftment; 25 = 25 days after engraftment.