Skip to main content
. 2001 Jun;69(6):4027–4033. doi: 10.1128/IAI.69.6.4027-4033.2001

FIG. 3.

FIG. 3

Type III secretion of EspG into supernatants and translocation into HEp-2 cells. Western blots with EspG antiserum indicate that EspG is produced by EPEC E2348/69 espG (pCVD453), EPEC E2348/69 escN (pCVD453), and in lesser amounts by wild-type EPEC E2348/69. EspG is observed in the supernatant and in the Triton X-100-soluble fraction of HEp-2 cells infected with EPEC E2348/69 espG (pCVD453) but not EPEC E2348/69 escN (pCVD453), indicating that secretion and translocation are dependent on type III secretion. Intimin is observed in whole cells but not in the Triton X-100-soluble fraction, indicating that the Triton X-100-soluble fraction is not contaminated with bacteria, and so EspG present in that fraction must be due to translocation and not contamination.