TABLE 2.

Virulence properties of wild-type and mutant EPEC strains

Strain	Results for assay of:
	LAa	FASb	Tir, EspABD secretionc	HEp-2 invasion (%)d	Intracellular persistence (%)e
E2348/69	+	+	+	100	100
E2348/69 espG	+	+	+	83	83
E2348/69 espG (pCVD453)	+	+	+	nt	nt
E2348/69 espG orf3	+	+	ntf	70	70

^aLA, localized adherence. 

^bFAS, fluorescent actin staining (an assay for attaching and effacing lesion formation). 

^cType III secretion of EspADB and Tir. 

^dInvasion of HEp-2 cells, relative to E2348/69. 

^eIntracellular persistence within HEp-2 cells after invasion, relative to E2348/69. 

^fnt, not tested.