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. 2023 Jan 4;16:1011895. doi: 10.3389/fnins.2022.1011895

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Copyright © 2023 Tian, Cook and Johnson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distortion and tissue tearing in light sheet microscopy (LSM) compared to magnetic resonance histology (MRH). A comparison between LSM images of a mouse brain stained with NeuN (A,C,E,F) and a diffusion weighted MRH image of the same specimen (B,D,G,H) highlights some of the challenges and opportunities. Red arrows indicate the tissue tearing. Purple arrows indicate the swelling (specimen 200316). Scale bar: 1 mm.