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. Author manuscript; available in PMC: 2023 Sep 1.
Published in final edited form as: Mol Psychiatry. 2022 May 12;27(9):3795–3805. doi: 10.1038/s41380-022-01610-x

Fig 2: SD triggers changes in MS oscillations and induces SIG through MS-DH projections.

Fig 2:

A Top, experimental design of LFP recordings performed during the pre- and post-stress retrieval tests. Middle, stress- and context-dependent changes of MS oscillatory activity. n=5 mice in each group. Middle Left: Two-Way RM ANOVA. Factor: Stress, F (1, 4) = 2.824, p=0.1682. Factor: Context, F (1, 4) = 19.65. p=0.0114. Factor: Context x Stress, F (1, 4) = 1.586. p=0.2764. Middle Center: Two-Way RM ANOVA. Factor: Stress, F (1, 4) = 13.93, p=0.0203. Factor: Context, F (1, 4) = 13.76. p=0.0207. Factor: Context x Stress, F (1, 4) = 4.104. p=0.1127. Middle Right: Two-Way RM ANOVA. Factor: Stress, F (1, 4) = 32.04, p=0.0048. Factor: Context, F (1, 4) = 62.88. p=0.0014. Factor: Context x Stress, F (1, 4) = 0.07422. p=0.7988, n=5 mice per group. Bottom, lack of changes in the directional coherence of MS and DH oscillatory activity. Bottom Left: Two-Way RM ANOVA. Factor: Stress, F (1, 4) = 0.3707, p=0.5755. Factor: Context, F (1, 4) = 0.1461. p=0.7218. Factor: Context x Stress, F (1, 4) = 0.6957. p=0.4512. Bottom Center: Two-Way RM ANOVA. Factor: Stress, F (1, 4) = 0.1235, p=0.7430. Factor: Context, F (1, 4) = 0.07882. p=0.7928. Factor: Context x Stress, F (1, 4) = 0.3309. p=0.5959. Bottom Right: Two-Way RM ANOVA. Factor: Stress, F (1, 4) = 0.7412, p=0.4378. Factor: Context, F (1, 4) = 0.008319. p=0.9317. Factor: Context x Stress, F (1, 4) = 0.09754. p=0.7704. B Visualization of MS neurons and MS to DH projections after injection of Cre-dependent AAV-GFP in Chat-Cre mice. C Top, localization of inhibitory DREADD and CNO infusions used for inhibition of MS to DH cholinergic projections by combining AAV-hM4Gi expression in MS to DH projections with injection of CNO in the DH. Cannulation was performed 72 hours before behavioral tests. Middle, experimental design depicting the timing of different circuit and behavioral manipulations. Bottom, reduced generalization of freezing in SD mice injected into DH with CNO. Bottom Left: Two-way RM ANOVA. Factor: Drug, F (1, 12) = 10.41, p=0.0073. Factor: Context, F (1, 12) = 20.03, p=0.0008. Factor: Drug x Context, F (1, 12) = 8.858, p=0.0116. n = 6 mice in Veh group, n = 8 mice in CNO group. Bottom Right: Mann Whitney Test. Sum of Ranks: 66 (Veh), 39 (CNO). p=0.0043.