Table 6.

Use of main alternative cellular models in parallel to PC12 cells in AD research.

Organism	Cell line	Tissue	Disease	Cell type	The expression of receptors													Number of articles		Reference
					nAChRs								mAChRs					Single number	Total number
					α3 nAChR	α4 nAChR	α5 nAChR	α6 nAChR	α7 nAChR	β1 nAChR	β2 nAChR	β4 nAChR	M1 mAChR	M2 mAChR	M3 mAChR	M4 mAChR	M5 mAChR
Homo sapiens	SH-SY5Y	bone marrow	neuroblastoma						√									71	136	Lukas et al., 1993; Peng et al., 1994
	HEK293	embryonic kidney			√	√			√									35		Kobayashi et al., 2013; Gong et al., 2016
	SK-N-SH		neuroblastoma											√		√		16		Baumgold and White, 1989 Ashkenazi et al., 1989
	HeLa	cervix	adenocarcinoma	epithelial			√			√			√	√				14		Pepitoni et al., 1991; Calleja-Macias et al., 2009
Cricetulus griseus	CHO	ovary											√					14	14	Mullaney et al., 1993
Mus Musculus	Neuro-2a	brain	neuroblastoma	neuroblast		√		√			√							11	11	Xiao et al., 2011; Srinivasan et al., 2012
Rattus norvegicus	C6	brain	glioma	glial cell		√			√		√	√						11	11	Wang and Tang, 2007; Niranjan et al., 2012; Terpinskaya et al., 2021
Cercopithecus aethiops	COS7	kidney		SV40 transformed	√	√					√			√				6	8	Neff et al., 1995; Kobayashi et al., 2013
	COS	kidney		SV40 transformed										√				2		Pals-Rylaarsdam et al., 1997; Vögler et al., 1998

In accordance with organism classification, the table characterizes the use of main alternative cellular models in parallel to PC12 cells. It is divided into five parts. These parts mainly include species, cell line, cell type, and number.

CHO, Naive Chinese hamster ovary; SV40, Simian Virus 40.