TABLE 1.
Effect of PbCHT1 knockout on P. berghei infectivity to A. stephensi
| Expt | Type of feed (clone)a | Mean ± SEM no. of oocysts (no. of midguts dissected)b in mosquitoes infected with:
|
% of WT value | |
|---|---|---|---|---|
| WT | PbCHT1-KO | |||
| 1 | gct (33) | 95.2 ± 5.8 (99) | 63.0 ± 3.9c (150) | 66 |
| 2 | gct (37) | 96.0 ± 6.3 (125) | 68.2 ± 4.0c (147) | 71 |
| 3 | gct (33) | 54.9 ± 4.5 (130) | 28.2 ± 2.5c (133) | 51 |
| 4 | gct (37) | 49.6 ± 3.0 (142) | 25.1 ± 2.2c (150) | 50 |
| 5 | ook (33) | 49.2 ± 2.4 (107) | 14.1 ± 0.9c (109) | 29 |
| 6 | ook (33) | 33.8 ± 1.7 (137) | 7.3 ± 0.6c (115) | 22 |
| 7 | ook (33) | 2.41 ± 0.2 (90) | 0.34 ± 0.06c (85) | 14 |
a
gct, gametocyte; ook, ookinete.
b
Each experiment included pooled data from three mice (gct) and three membrane feeders (ook).
c
Significantly different (P < 0.01) from the value for the WT-infected control group, as calculated by Student's t test.