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. 2023 Jan 4;12:946320. doi: 10.3389/fonc.2022.946320

Figure 2.

Figure 2

Inferred COSMro network differentiating radiation resistant rSCC-61 from radiation sensitive SCC-61 Cells. (A) rSCC-61 specific network inferred by COSMro model. Measured and inferred activity of signaling proteins and inferred direction of metabolic reactions is presented with four radiation resistant network signatures highlighted. (B) Akt subnetwork showing predicted upregulation of Akt2 and β-catenin and downregulated pGSK3β in radiation resistant rSCC-61 cells. (C) Western blot analysis of SCC-61 and rSCC-61 cell lysates with antibodies against pAkt (T308), pAkt2 (S474), pGSK3β (S9), and GAPDH as a loading control to validate network prediction. Quantification is shown on the right panels. (D) RT-PCR analysis showing increased expression of β-catenin in rSCC-61 compared to SCC-61 (left) and mRNA data extracted from the previously reported Illumina transcriptomic data (right). Primers are summarized in Supplementary Table S4 . p values *0.05-0.01, **0.01-0.001, ***<0.001 (n = 3).