TABLE 3.

Coronavirus disease-2019 (COVID-19) polymerase chain reaction (PCR) test kits affected by primer nucleotide substitutions.

COVID-19 PCR test kit	PCR primer target in SARS-CoV-2 genome	PCR primer type	PCR primer nucleotide substitution	Number of genomes with PCR primer nucleotide substitution
China CDC	N	Forward	C28887T	20
China CDC	N	Forward	G28881T	5
China CDC	N	Forward	G28882T	1
US CDC	N1	P	C28310T	12
US CDC	N3	Forward	A28699G	2
Charite	RdRp	Forward	G15451A	4
Charite	E	Forward	A26271G	3
Charite	N	P	C28775T	1
Pasteur	RdRp	Reverse	C14184T	1

N, nucleocapsid, RdRp, RNA dependent RNA polymerase.