Figure 6.

S1 contains a cryptic, Mn²⁺-sensitive adhesive epitope.A, FN-null MEFs (9.4 × 10⁴ cells/cm²) were seeded on wells precoated with 250 nM reduced or nonreduced S1 protein, S1-RBD, or 1% bovine serum albumin (BSA). Cells were seeded for 1 h in the absence or presence of 1 mM MnCl₂ or 10 mM EDTA. A, representative images of crystal violet–stained cells. The scale bar represents 100 μm. B, relative cell number presented as mean absorbance ± SEM, n = 3 independent experiments performed in triplicate. ∗p < 0.05 versus corresponding BSA control by two-way ANOVA with Bonferroni’s post hoc test.