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. 2023 Jan 18;290(1991):20221766. doi: 10.1098/rspb.2022.1766

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© 2023 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — Variant allele frequencies (VAFs) for parents and their respective sperm pools. (a) GoH mutations for which sperm VAF =0 were classified as parent only (black), likewise LoH variants in the parent for which the VAF < 1 in the sperm were classified as parent only. All other SNVs were classified as being shared by both a parent branch and its corresponding sperm pool (red). (b) For GoH SNVs that are shared by a parent branch and its sperm, average VAF of the two replicate parent libraries is weakly correlated with the average VAF of the two replicate sperm pool libraries. The slope of the relationship is 0.36 and R² = 0.081 (trendline shown in red). The black line shows a 1 : 1 line for reference.