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Detection of EspD, EspA, and EspB in EPEC supernatants. DMEM culture supernatants were analyzed by using Western blotting. Wild-type EPEC E2348/69 (lane 1), UMD870(pLCL123) (lane 3), UMD870(pICC72) (lane 4), UMD870(pICC73) (lane 5), and UMD870(pICC74) (lane 6) all demonstrated similar levels of the secreted proteins. EspD was absent from the supernatant of the espD deletion mutant strain UMD870 (lane 2), while UMD870 had reduced supernatant levels of EspA and normal levels of EspB.
